Fig. 3. HIF-1α protein accumulation and VEGF expression in cultured hypoxic MIO-M1 cells. (A) Exposure of MIO-M1 cells to 1% O₂ hypoxia (indicated time) induces HIF-1α protein stability. (B-E) Hypoxic conditions lead to increase expression of VEGF and ANGPTL-4 mRNA and protein. All the samples were normalized to their β-actin expression (n=4 per group). Data represents means ± SD of relative values vs control from 3 independent experiments. *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001, statistical analysis was performed with one-way ANOVA with Dunn's test for multiple comparisons.